In this workshop, we will provide basics to advanced training of all the tools, techniques, and methodologies used to learn the Bioinformatics, Genomics, Next Generation Sequencing (NGS) data analysis. After this workshop, participant will be perfectly ready to start Master's degree/PhD/Postdoctoral research in the areas mentioned above. This workshop will provide a platform for faculty and researchers to improve their skills by updating themselves with latest methodologies in the area. We will cover basics to advanced aspects of all the topics mentioned below. 

 

Who can participate: Students (B.Sc./B.Tech/M.Sc./M.Tech), Research Fellows (JRF/SRF/RA etc.), PhD students, Faculty, Scientists, and Company Personals. 

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Internship Logistics 

Last date of registration: March 11, 2023.

Fee: Rs. 960/- for Indian Participants, $25 (USD) for International Participants.

Timings: 07:00 PM - 08:00 PM Indian Standard Time (IST). Every session will be 60 minutes duration.

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Certification and other highlights

• Participants must have access to the laptop/desktop with a stable internet connection. 

• Participants successfully completing the internship will be provided with E-certificate.

• The entire internship will be online and interactive (~60 Hours).

• We will provide the full recordings of all the internship sessions. 

• We will provide the link (e.g. Webex) to join the training sessions, 

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Help and Support 

support@nextgenhelper.com

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Workshop Timings (Indian Standard Time e.g. IST)

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March 13, 2023 (07:00 - 08:00 PM): Setup of Linux system for Bioinformatics

• Start working on Linux System

• Basic Linux commands

 

March 14, 2023 (07:00 - 08:00 PM): Basic Linux commands and file handling​

• Basic commands to play with data

• File Handeling 

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March 15, 2023 (07:00 - 08:00 PM): Basics of 'R'

• Basic operation with R

• Data analysis in R

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March 16, 2023 (07:00 - 08:00 PM): Advanced commands in 'R'

• File handling in R and Graph Preparation

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March 17, 2023 (07:00 - 08:00 PM): Basics of Next Generation Sequencing (NGS)

• Data generation in NGS technologies

• NGS data file handeling 

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March 18, 2023 (07:00 - 08:00 PM): Quality Control of Next Generation Sequencing (NGS) datasets

• Basics of NGS datasets, generation and Quality Score.

• Data handling and Filtering of NGS datasets, trimming etc.

• Training of tools to filter the NGS datasets.

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March 19, 2023 : You have to Practice, No live session.

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March 20, 2023 (07:00 - 08:00 PM): NGS data analysis (Alignment & Variant Calling)- I

• SNP basics

• SNP identification using samtools

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March 21, 2023 (07:00 - 08:00 PM): NGS data analysis (Alignment & Variant Calling)- II

• SNP identification using freebayes

• Filtration and annotation of SNPs.

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March 22, 2023 (07:00 - 08:00 PM): Microbiome data analysis -I 

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March 23, 2023 (07:00 - 08:00 PM): Microbiome data analysis -II

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March 24, 2023 (07:00 - 08:00 PM): You have to Practice, No live session.

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March 25, 2023 (07:00 - 08:00 PM): Gene expression with RNA-Seq data analysis -I

• Introduction to RNA Seq- a quick recap

• Indexing of reference genome.

• Generation of BAM file and its index generation.

• Alignment matrices of the mapped BAM file.

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March 26, 2023 (07:00 - 08:00 PM): Gene expression with RNA-Seq data analysis -II

• Quantification tools and generation of count matrix.

• Introduction to Gene Expression.

• Differential gene expression tools and their algorithms.

• Differential gene expression analysis using DESeq2 R package.

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Scientists/Resource persons for this internship